The goalkeeper in question saved two spot-kicks to help her country to qualify for the 2022 Women's Asia Cup for the first time in their history.
Iranian women's goalkeeper, Zohreh Koudaei has been accused of lying that she's a woman.
The president of the Jordan Football Association (JFA) made the accusation calling on the Asian Football Confederation (AFC) to carry out a gender test on Koudaei.
Iran beat Jordan 4-2 on penalties in September, with Koudaei saving two spot-kicks to help her country to qualify for the 2022 Women's Asia Cup for the first time in their history.
But the JFA have doubts over the 'eligibility of a participating player,' and are demanding a gender test on the shot-stopper. They believe Koudaei was in fact a man impersonating a woman.
In response, Iran's team selector Maryam Irandoost strongly denied the allegations.
Irandoost told sports news site Varzesh3: "The medical staff have carefully examined each player on the national team in terms of hormones to avoid any problems in this regard, and so I tell all fans not to worry.
"We will provide any documentation that the Asian Confederation of Football wishes without wasting time."
It comes after the President of the Jordan Football Association, Prince Ali Bin Al-Hussein, tweeted a letter calling for a gender test.
He has slammed the AFC over the 'very serious issue,' and fumed at the confederation to 'wake up'.
The JFA have demanded an 'independent panel of medical experts' take a look at the issue.
Part of the letter reads that the JFA: "Understand that gender verification is not mandatory for participating players, however, the article reserves the right for AFC to investigate and take appropriate measures if there is a doubt on the eligibility of a participating player.
"Considering the evidence submitted by JFA and given the importance of the competition,
"We request the AFC to initiate a transparent and clear investigation by a panel of independent medical experts to investigate the eligibility of the player in question and others on the team,
"Particularly that the Iranian Women Football team has a history with gender and doping issues."