The 86-year-old also warned the 10-month-old conflict is increasing food shortages around the world, urging an end to the use of "food as a weapon".
Pope Francis has called for an end of what he called a 'senseless' war in Ukraine.
The Leader of the Roman Catholic church, Pope Francis on Sunday December 25, called on Russia to stop killing people in Ukraine during his traditional Christmas message from St Peter's Basilica at the Vatican.
The 86-year-old also warned the 10-month-old conflict is increasing food shortages around the world, urging an end to the use of "food as a weapon".
He addressed thousands of faithful gathered in St Peter's Square, some of them holding Ukrainian flags, before delivering the "Urbi et Orbi" blessing ("to the city and the world").
He has repeatedly called for peace ever since Russia invaded Ukraine in February, condemning the war but seeking to maintain a delicate dialogue with Moscow.
In his address he said; "our Ukrainian brothers and sisters who are experiencing this Christmas in the dark and cold, far from their homes".
"May the Lord inspire us to offer concrete gestures of solidarity to assist all those who are suffering, and may he enlighten the minds of those who have the power to silence the thunder of weapons and put an immediate end to this senseless war!"
"Tragically, we prefer to heed other counsels, dictated by worldly ways of thinking", he added, recalling "with sorrow" that "the icy winds of war continue to buffet humanity".
"Our time is experiencing a grave famine of peace also in other regions and other theatres of this third world war," he said.
He referenced numerous countries in difficulty this Christmas, from Afghanistan to Yemen, Syria, Myanmar, the Israeli-Palestinian conflict, Lebanon and Haiti.
For the first time, he also called for "reconciliation" in Iran, rocked by women-led protests for the past three months.
The pope then urged those celebrating Christmas to remember all those "who go hungry while huge amounts of food daily go to waste and resources are being spent on weapons".
"The war in Ukraine has further aggravated this situation, putting entire peoples at risk of famine, especially in Afghanistan and in the countries of the Horn of Africa," he said.
"We know that every war causes hunger and exploits food as a weapon, hindering its distribution to people already suffering.
"On this day, let us learn from the Prince of Peace (Jesus Christ) and, starting with those who hold political responsibilities, commit ourselves to make food solely an instrument of peace."